Detection of Ru potential metallodrug in human urine by MALDI-TOF mass spectrometry: Validation and options to enhance the sensitivity

We studied the possibility of detection [Ru(η5-C5H5)(PPh3)2Cl] (abbreviated by RuCp) complex as a model system for Ru-based metallodrugs in human urine using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) without previous purification or removal inorganic salts. Inorganic salts might prevent RuCp MALDI-TOF MS, most likely through increased number and intensity background/organic matrix signals. This problem be overcome acquisition matrix-free spectra addition nanoparticles, such carbon dots, to solution. Our results suggest that is easily detectable MS all conditions, with CHCA being best phosphate-containing solutions, whereas urine, DHB approach demonstrated highest sensitivity, precision, reproducibility. The sensitivity MALDI could dots urine. Based on theoretical calculations matrix/analyte combinations, interaction was established, higher explained.